Impact of waste types and associated houseflies in circulation of the hemorrhagic bacteria Escherichia coli O157: H7 in different districts in Giza governorate, Egypt

Document Type : Original Article

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Department of Entomology, faculty of Science, Ain Shams University, Cairo, Egypt

Abstract

Escherichia coli O157: H7 (STEC) was isolated from eight sites of different waste types and their associated flies, in Giza governorate. Wastes as breading sites for houseflies were sorted according to their types and contents to 4 types:Slaughter type, Agricultural, General and Household type. Bacterial isolation from general type waste sites which contained food remainders, diapers, rotten vegetables and human excreta, showed the highest prevalence of Escherichia coli O157: H7 for wastes and associated flies recording 10% and 6.6% as prevalence percentage of the bacteria.Two sites were found sharing STEC from waste components and associated flies which are sites (four and seven). The amplification of the gene region of 16S rRNAof Escherichia coli O157: H7 as well as the detection of shiga like toxins stx1, stx2 and eae genes were shown positive for the bacterial isolates number (4 and 7) when studied using molecular PCR amplification techniques.

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